alpha-Mannosidosis Mutation Database
A database on mutations, genotypes and the clinical and molecular aspects of alpha-Mannosidosis
Patients and Genotypes
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Western blot analysis, transfected COS-7 cells
Cells were transfected with the appropriate MAN2B1-construct or empty vector and harvested 36-48 h post-transfection. Ten μg of protein (lysate) or 10 μl of medium was analysed by SDS/PAGE (4–12% gel) under reducing conditions and electroblotted onto a PVDF membrane. MAN2B1 was detected using antiserum against native recombinant human MAN2B1 and ALP-conjugated secondary antibody followed by chemiluminescence detection.
Kuokkanen et al. 2011