alpha-Mannosidosis Mutation Database
A database on mutations, genotypes and the clinical and molecular aspects of alpha-Mannosidosis
Patients and Genotypes
Access unpublished data
Western blot analysis, transfected BHK-21 cells
Cells were transfected with the appropriate MAN2B1-construct or empty vector and harvested 36-48 h post-transfection. Ten μg of protein (lysate) or 10 μl of medium was analyzed by SDS/PAGE (4–12% gel) under reducing conditions and electroblotted onto a PVDF membrane. MAN2B1 was detected using antiserum against native recombinant human MAN2B1 (Zymenex) and ALP-conjugated secondary antibody (Santa Cruz) followed by chemiluminescence detection.
( Unpublished )